Chemistry Department Seminar: Antoine van Oijen, Groningen University
The Jerry and Jean Mohrig Lecture
Under the hood: Single-molecule studies of DNA replication
Antoine van Oijen
Novel nanomanipulation and imaging methods have made it possible to study biochemical reactions at the level of individual proteins. In a biological context, most of these proteins function in concert with others in multi-protein complexes, so an important future direction is the utilization of single-molecule techniques to unravel the orchestration of large macromolecular assemblies. I will discuss our single-molecule studies of the replisome, the multi-protein machinery that is responsible for replication of DNA. I will present experiments that rely on the readout of mechanical DNA properties as well as single-molecule fluorescence imaging to obtain information on the catalytic activity and dynamic composition of the bacterial replisome. Further, I will discuss new results on the study of individual eukaryotic replisomes in Xenopus cellular extracts.
Sponsored by Chemistry. Contact: Gretchen Hofmeister, x7203