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Chemistry Department Seminar: Antoine van Oijen, Groningen University

Date: Friday, April 20th, 2012

Time: 3:30 pm

Duration: 1 hour

Location: Olin 04

Contact: Gretchen Hofmeister, x7203

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The Jerry and Jean Mohrig Lecture

Under the hood:  Single-molecule studies of DNA replication

Antoine van Oijen
Groningen University

Novel nanomanipulation and imaging methods have made it possible to study biochemical reactions at the level of individual proteins.  In a biological context, most of these proteins function in concert with others in multi-protein complexes, so an important future direction is the utilization of single-molecule techniques to unravel the orchestration of large macromolecular assemblies.  I will discuss our single-molecule studies of the replisome, the multi-protein machinery that is responsible for replication of DNA.  I will present experiments that rely on the readout of mechanical DNA properties as well as single-molecule fluorescence imaging to obtain information on the catalytic activity and dynamic composition of the bacterial replisome.  Further, I will discuss new results on the study of individual eukaryotic replisomes in Xenopus cellular extracts.


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